Hotspot

rbcL

LSC · 58411-59859 · 1449 bp

Back to Pleuropterus

Pi 0

MarkerSeek score 29.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1449
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGT	45.0	40.0	57.0	54.4	1553 bp	1.0	43.4
rbcL_p2	ACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGTT	45.0	38.1	57.0	55.2	1553 bp	1.0	42.2
rbcL_p3	AACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGT	42.9	40.0	57.8	54.4	1554 bp	1.0	41.2
rbcL_p4	ACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGTTT	45.0	36.4	57.0	56.0	1553 bp	1.0	40.4
rbcL_p5	AACGCCTAATTCATGTCGAGT	AGTAAAAGATTGAGCCGAGTT	42.9	38.1	57.8	55.2	1554 bp	1.0	40.0