Hotspot

atpF

LSC · 13104-14414 · 1311 bp

Back to Pleuropterus

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1311
variable_sites: 2
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTCATCAGCTCGAAGGGTTG	AGTTTTTGAATCCATTTTCGA	50.0	28.6	57.9	52.2	1513 bp	1.0	40.1
atpF_p2	TTCATCAGCTCGAAGGGTTG	AGGAAATAGGTCAAGTAATACAACA	50.0	32.0	57.9	56.0	1483 bp	1.0	40.1
atpF_p3	TTCATCAGCTCGAAGGGTTG	AGTTTTTGAATCCATTTTCGAT	50.0	27.3	57.9	52.9	1513 bp	1.0	40.1
atpF_p4	TTCATCAGCTCGAAGGGTTG	AGTTTTTGAATCCATTTTCGATT	50.0	26.1	57.9	53.7	1513 bp	1.0	40.1
atpF_p5	GCTCGAAGGGTTGTCATGAG	AGTTTTTGAATCCATTTTCGA	55.0	28.6	58.6	52.2	1506 bp	1.0	40.1