Hotspot

rps15-ycf1

IRa · 132744-133035 · 292 bp

Back to Pleuropterus

Pi 0.084

MarkerSeek score 73.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: IRa
length_bp: 292
variable_sites: 21
pi: 0.084
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAGTCGTTGACGTTTTCCCA	GGATCCAATGCTAATCCGGG	45.0	55.0	58.0	58.2	1058-1145 bp	1.0	83.5
rps15-ycf1_p2	AGTCGTTGACGTTTTCCCAA	GGATCCAATGCTAATCCGGG	45.0	55.0	58.0	58.2	1057-1144 bp	1.0	83.5
rps15-ycf1_p3	AAGTCGTTGACGTTTTCCCA	GATTATGCGCTGTCCGTACT	45.0	50.0	58.0	57.9	1150-1237 bp	1.0	83.4
rps15-ycf1_p4	AGTCGTTGACGTTTTCCCAA	GATTATGCGCTGTCCGTACT	45.0	50.0	58.0	57.9	1149-1236 bp	1.0	83.4
rps15-ycf1_p5	TAAGCCAGAAGTCGTTGACG	GATTATGCGCTGTCCGTACT	50.0	50.0	57.9	57.9	1158-1245 bp	1.0	83.1