Hotspot

rbcL-accD

LSC · 59860-60344 · 485 bp

Back to Pleuropterus

Pi 0.00619

MarkerSeek score 59.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 3
pi: 0.00619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGGAGTCCTGAGCTAGCT	TAGCCCACACCTATGTTCGA	50.0	50.0	57.8	58.1	628 bp	1.0	77.8
rbcL-accD_p2	TCGTAAAGCTGCCAAATGGA	TAGCCCACACCTATGTTCGA	45.0	50.0	57.8	58.1	642 bp	1.0	77.7
rbcL-accD_p3	CTGCCAAATGGAGTCCTGAG	TAGCCCACACCTATGTTCGA	55.0	50.0	58.3	58.1	634 bp	1.0	77.2
rbcL-accD_p4	CGTAAAGCTGCCAAATGGAG	TAGCCCACACCTATGTTCGA	50.0	50.0	57.5	58.1	641 bp	1.0	74.8
rbcL-accD_p5	GCCGCTTGTGAAGTATGGAA	TAGCCCACACCTATGTTCGA	50.0	50.0	58.6	58.1	608 bp	1.0	74.8