Hotspot

rps15-trnL

SSC · 100168-104495 · 4328 bp

Back to Pleuropogon

Pi 0.0238

MarkerSeek score 63.60

Primer available yes

Label rps15-trnL

Feature Metadata

label_name: rps15-trnL
feature_type: igs
region: SSC
length_bp: 4328
variable_sites: 170
pi: 0.0238
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnL_p1	AACACCCATCAATGCATGGT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.1	58.0	387-403 bp	0.75	81.2
rps15-trnL_p2	ACCCATCAATGCATGGTGTT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.1	58.0	384-400 bp	0.75	81.2
rps15-trnL_p3	AATCGCGGAAAACCGAAGTA	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	57.9	58.0	281-297 bp	0.75	81.0
rps15-trnL_p4	TCGCGGAAAACCGAAGTATT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	57.9	58.0	279-295 bp	0.75	81.0
rps15-trnL_p5	ACCCATCAATGCATGGTGTT	GCCTATCCTCATGAAACGGG	45.0	55.0	58.1	58.1	898-914 bp	0.75	76.1