Hotspot

petA-psbJ

LSC · 59468-60293 · 826 bp

Back to Pleuropogon

Pi 0.0311

MarkerSeek score 72.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 42
pi: 0.0311
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAATTGTACGA	TCCTCTTTGGCTGATAGGTACT	33.3	45.5	53.7	58.0	940-966 bp	1.0	45.6
petA-psbJ_p2	TGAAAAGGTTCAATTGTACGA	CCTCTTTGGCTGATAGGTACTG	33.3	50.0	53.7	57.9	939-965 bp	1.0	45.6
petA-psbJ_p3	AGAAACAGTTTGAAAAGGTTCA	TCCTCTTTGGCTGATAGGTACT	31.8	45.5	54.6	58.0	950-976 bp	1.0	45.6
petA-psbJ_p4	AGAAACAGTTTGAAAAGGTTCA	CCTCTTTGGCTGATAGGTACTG	31.8	50.0	54.6	57.9	949-975 bp	1.0	45.6
petA-psbJ_p5	TGAAAAGGTTCAATTGTACGAA	TCCTCTTTGGCTGATAGGTACT	31.8	45.5	54.5	58.0	940-966 bp	1.0	45.6