Hotspot

rbcL-petA

LSC · 56220-58504 · 2285 bp

Back to Pleuropogon

Pi 0.0181

MarkerSeek score 57.90

Primer available yes

Label rbcL-petA

Feature Metadata

label_name: rbcL-petA
feature_type: igs
region: LSC
length_bp: 2285
variable_sites: 68
pi: 0.0181
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-petA_p1	TCGAGTTCGAGCCAGTAGAT	TCTGTCCTCTTCCTCGGTTT	50.0	50.0	58.0	58.0	2853-2897 bp	0.5	61.5
rbcL-petA_p2	TCGAGTTCGAGCCAGTAGAT	CGAATCCCTCCGGCAAAATA	50.0	50.0	58.0	58.0	2622-2666 bp	0.5	61.4
rbcL-petA_p3	TCGAGTTCGAGCCAGTAGAT	GGAACAGGGCCTATCACAAG	50.0	55.0	58.0	58.0	2734-2778 bp	0.5	61.3
rbcL-petA_p4	TCTTCACATTCACCGTGCAA	TTGCTTCTCGTGGGTTTTCA	45.0	45.0	58.0	58.0	2954-2998 bp	0.5	61.1
rbcL-petA_p5	TTCTTCACATTCACCGTGCA	TTGCTTCTCGTGGGTTTTCA	45.0	45.0	58.0	58.0	2955-2999 bp	0.5	61.1