Hotspot

psaA-ycf3

LSC · 41767-42374 · 608 bp

Back to Pleuropogon

Pi 0.0285

MarkerSeek score 70.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 30
pi: 0.0285
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	804-805 bp	1.0	80.2
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	771-772 bp	1.0	79.7
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	GGTTGAAGATTACGAAGCGC	50.0	50.0	58.1	57.6	728-729 bp	1.0	79.2
psaA-ycf3_p4	CTGGCCCATTCCTCAAAAGA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	802-803 bp	1.0	79.0
psaA-ycf3_p5	TCTGGCCCATTCCTCAAAAG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	803-804 bp	1.0	79.0