Hotspot

psbM-trnC

LSC · 17020-18661 · 1642 bp

Back to Pleuropogon

Pi 0.0176

MarkerSeek score 67.80

Primer available yes

Label psbM-trnC

Feature Metadata

label_name: psbM-trnC
feature_type: igs
region: LSC
length_bp: 1642
variable_sites: 49
pi: 0.0176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnC_p1	TGCTACTGCACTGTTCATTCT	AGTTCAAATCTGGGTGCCG	42.9	52.6	57.6	58.1	1667-1808 bp	1.0	66.5
psbM-trnC_p2	TGCTACTGCACTGTTCATTCT	CCAGTTCAAATCTGGGTGCC	42.9	55.0	57.6	59.1	1669-1810 bp	1.0	66.1
psbM-trnC_p3	TGCTACTGCACTGTTCATTCT	CAGTTCAAATCTGGGTGCCG	42.9	55.0	57.6	59.5	1668-1809 bp	1.0	63.2
psbM-trnC_p4	TGCTACTGCACTGTTCATTC	AGTTCAAATCTGGGTGCCG	45.0	52.6	56.1	58.1	1667-1808 bp	1.0	62.4
psbM-trnC_p5	TGCTACTGCACTGTTCATTCT	AGGGGACTGCAAATCCTTTA	42.9	45.0	57.6	56.4	1692-1833 bp	1.0	62.2