Hotspot

trnT-trnE

LSC · 15004-15502 · 499 bp

Back to Pleuropogon

Pi 0.019

MarkerSeek score 65.20

Primer available yes

Label trnT-trnE

Feature Metadata

label_name: trnT-trnE
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 17
pi: 0.019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnE_p1	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	589-620 bp	1.0	73.4
trnT-trnE_p2	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	568-599 bp	1.0	73.3
trnT-trnE_p3	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	552-583 bp	1.0	73.3
trnT-trnE_p4	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	531-562 bp	1.0	73.3
trnT-trnE_p5	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	557-588 bp	1.0	72.5