Hotspot

tRNA-Gly-trnT

LSC · 13361-14931 · 1571 bp

Back to Pleuropogon

Pi 0.0269

MarkerSeek score 69.20

Primer available yes

Label tRNA-Gly-trnT

Feature Metadata

label_name: tRNA-Gly-trnT
feature_type: igs
region: LSC
length_bp: 1571
variable_sites: 74
pi: 0.0269
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gly-trnT_p1	ACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	38.1	50.0	55.5	58.0	1715-1772 bp	1.0	57.8
tRNA-Gly-trnT_p2	AGAACGAATCACACTTTTACCAC	ACGTCTTACCACGGCATTAC	39.1	50.0	57.3	58.0	1718-1775 bp	1.0	56.7
tRNA-Gly-trnT_p3	GAACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	39.1	50.0	57.3	58.0	1717-1774 bp	1.0	56.7
tRNA-Gly-trnT_p4	AACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	36.4	50.0	56.2	58.0	1716-1773 bp	1.0	55.9
tRNA-Gly-trnT_p5	ACGAATCACACTTTTACCACT	CGTCTTACCACGGCATTACT	38.1	50.0	55.5	57.7	1714-1771 bp	1.0	55.5