Hotspot

psbZ-trnG

LSC · 11653-11911 · 259 bp

Back to Pleuropogon

Pi 0.0341

MarkerSeek score 69.30

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 13
pi: 0.0341
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	50.0	50.0	57.8	57.6	291-427 bp	1.0	81.6
psbZ-trnG_p2	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	55.0	50.0	58.3	57.6	289-425 bp	1.0	81.2
psbZ-trnG_p3	TTGCTTCTCCTGATGGTTGG	CCGTATCTTCTCCTTGGCAA	50.0	50.0	57.8	57.3	285-421 bp	1.0	79.6
psbZ-trnG_p4	GCTTCTCCTGATGGTTGGTC	CCGTATCTTCTCCTTGGCAA	55.0	50.0	58.3	57.3	283-419 bp	1.0	79.2
psbZ-trnG_p5	TTGCTTCTCCTGATGGTTGG	GGGAATCGAACCCGTATCTT	50.0	50.0	57.8	57.1	296-432 bp	1.0	77.8