Hotspot

psbI-trnS

LSC · 7346-7465 · 120 bp

Back to Pleuropogon

Pi 0.0486

MarkerSeek score 78.00

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 10
pi: 0.0486
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	ATCCAATGACCCAGGACGTA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.1	58.0	228-229 bp	1.0	82.2
psbI-trnS_p2	TATCCAATGACCCAGGACGT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.1	58.0	229-230 bp	1.0	82.2
psbI-trnS_p3	ATCCAATGACCCAGGACGTA	CTTTCGATTTGGAACGGGGA	50.0	50.0	58.1	58.2	265-266 bp	1.0	80.5
psbI-trnS_p4	ATCCAATGACCCAGGACGTA	TTTCGATTTGGAACGGGGAG	50.0	50.0	58.1	58.2	264-265 bp	1.0	80.5
psbI-trnS_p5	TATCCAATGACCCAGGACGT	TTTCGATTTGGAACGGGGAG	50.0	50.0	58.1	58.2	265-266 bp	1.0	80.5