Hotspot

rps15-ycf1

SSC · 129281-129726 · 446 bp

Back to Pleurophora

Pi 0.0254

MarkerSeek score 57.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 446
variable_sites: 16
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	AACTCCATTGCAAGACAGCA	50.0	45.0	58.0	58.0	1403-1406 bp	1.0	81.1
rps15-ycf1_p2	GATGAGCCGAAGAACACCAT	AACTCCATTGCAAGACAGCA	50.0	45.0	58.0	58.0	1278-1281 bp	1.0	81.1
rps15-ycf1_p3	AACACCATGCATTGATGGGT	AACTCCATTGCAAGACAGCA	45.0	45.0	58.1	58.0	1266-1269 bp	1.0	80.9
rps15-ycf1_p4	AAGGATGAGCCGAAGAACAC	AACTCCATTGCAAGACAGCA	50.0	45.0	57.9	58.0	1281-1284 bp	1.0	80.6
rps15-ycf1_p5	ATGCATTGATGGGTGGTGAG	AACTCCATTGCAAGACAGCA	50.0	45.0	58.2	58.0	1260-1263 bp	1.0	79.6