Hotspot

ndhF-rpl32

SSC · 117814-118820 · 1007 bp

Back to Pleurophora

Pi 0.0146

MarkerSeek score 70.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1007
variable_sites: 22
pi: 0.0146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CACACGCGACGAAGATTTTT	CGCTGCCCAATATGCTTTTT	45.0	45.0	57.7	57.7	1197-1213 bp	1.0	78.3
ndhF-rpl32_p2	CACACGCGACGAAGATTTTT	AAAAAGCTTTCAACGCTGCC	45.0	45.0	57.7	58.4	1210-1226 bp	1.0	77.2
ndhF-rpl32_p3	ACTGGTAGTGGAATGAACGG	CGCTGCCCAATATGCTTTTT	50.0	45.0	57.2	57.7	1131-1147 bp	1.0	74.7
ndhF-rpl32_p4	CTGGTAGTGGAATGAACGGT	CGCTGCCCAATATGCTTTTT	50.0	45.0	57.2	57.7	1130-1146 bp	1.0	74.7
ndhF-rpl32_p5	CACACGCGACGAAGATTTTT	ACGCTGCCCAATATGCTTTT	45.0	45.0	57.7	58.8	1198-1214 bp	1.0	74.2