Hotspot

petA-psbJ

LSC · 67898-68866 · 969 bp

Back to Pleurophora

Pi 0.00894

MarkerSeek score 63.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 13
pi: 0.00894
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ACTCCGGGTTAGGTTCATCT	50.0	50.0	58.0	57.8	1114-1126 bp	1.0	78.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ACTCCGGGTTAGGTTCATCT	50.0	50.0	57.8	57.8	1097-1109 bp	1.0	77.7
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ACTCCGGGTTAGGTTCATCT	50.0	50.0	58.2	57.8	1111-1123 bp	1.0	77.5
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	TCCGGGTTAGGTTCATCTCT	50.0	50.0	58.0	57.5	1112-1124 bp	1.0	76.3
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	ACTCCGGGTTAGGTTCATCT	45.0	50.0	57.6	57.8	1109-1121 bp	1.0	76.1