Hotspot

rpoB-trnC-GCA

LSC · 28518-29737 · 1220 bp

Back to Pleurophora

Pi 0.0109

MarkerSeek score 66.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 20
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCGTCTGTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1381-1386 bp	1.0	77.5
rpoB-trnC-GCA_p2	AGTTCGTCTGTTAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1382-1387 bp	1.0	77.4
rpoB-trnC-GCA_p3	GTTCGTCTGTTAAGCCCTGA	TTGTTAATCAGGCGACACCC	50.0	50.0	57.5	58.2	1399-1404 bp	1.0	76.6
rpoB-trnC-GCA_p4	AGTTCGTCTGTTAAGCCCTG	TTGTTAATCAGGCGACACCC	50.0	50.0	57.5	58.2	1400-1405 bp	1.0	76.6
rpoB-trnC-GCA_p5	CGTCTGTTAAGCCCTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1378-1383 bp	1.0	75.7