Hotspot

rps16-trnQ-UUG

LSC · 6463-7906 · 1444 bp

Back to Pleurophora

Pi 0.0159

MarkerSeek score 64.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1444
variable_sites: 34
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1490-1512 bp	1.0	81.6
rps16-trnQ-UUG_p2	TCATGTCTGTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1506-1528 bp	1.0	81.4
rps16-trnQ-UUG_p3	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	1494-1516 bp	1.0	80.1
rps16-trnQ-UUG_p4	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.5	58.1	1496-1518 bp	1.0	79.5
rps16-trnQ-UUG_p5	GTCGCACGTTGCTTTTTACC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1493-1515 bp	1.0	78.3