Hotspot

trnK-UUU-rps16

LSC · 4427-5327 · 901 bp

Back to Pleurophora

Pi 0.0157

MarkerSeek score 63.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 20
pi: 0.0157
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGCGGGGGTTTTTACA	50.0	45.0	58.2	58.2	956-990 bp	1.0	82.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGCGGGGGTTTTTACA	50.0	45.0	58.2	58.2	955-989 bp	1.0	82.4
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	AAAAGGCGGGGGTTTTTACA	50.0	45.0	57.8	58.2	1041-1075 bp	1.0	81.4
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	AAAAGGCGGGGGTTTTTACA	50.0	45.0	57.8	58.2	1040-1074 bp	1.0	81.4
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	AAAAGGCGGGGGTTTTTACA	50.0	45.0	58.3	58.2	965-999 bp	1.0	81.4