Hotspot

trnP-UGG-psaJ

LSC · 66954-67443 · 490 bp

Back to Pleroma

Pi 0.00428

MarkerSeek score 63.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 2
pi: 0.00428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GCTAAAGACCCGAACCAGAG	50.0	55.0	58.1	58.0	590-613 bp	1.0	82.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GCTAAAGACCCGAACCAGAG	50.0	55.0	58.1	58.0	589-612 bp	1.0	82.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GCTAAAGACCCGAACCAGAG	50.0	55.0	58.1	58.0	601-624 bp	1.0	82.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GCTAAAGACCCGAACCAGAG	50.0	55.0	58.1	58.0	600-623 bp	1.0	82.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GCTAAAGACCCGAACCAGAG	50.0	55.0	58.1	58.0	603-626 bp	1.0	82.3