Hotspot

accD-psaI

LSC · 59683-60346 · 664 bp

Back to Pleroma

Pi 0.00519

MarkerSeek score 64.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 3
pi: 0.00519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	792-899 bp	1.0	70.5
accD-psaI_p2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	791-898 bp	1.0	70.5
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	798-905 bp	1.0	69.8
accD-psaI_p4	AAGGGTGTTCTGAGTGAGTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	797-904 bp	1.0	69.8
accD-psaI_p5	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	792-899 bp	1.0	68.6