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atpH-atpI

LSC · 13396-14333 · 938 bp

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Pi 0.00333

MarkerSeek score 64.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 3
pi: 0.00333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.0	57.9	977-1014 bp	1.0	81.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.0	58.1	989-1026 bp	1.0	81.8
atpH-atpI_p3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1007-1044 bp	1.0	81.5
atpH-atpI_p4	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1019-1056 bp	1.0	81.4
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.2	57.9	978-1015 bp	1.0	80.8