Hotspot

psbI-trnS-GCU

LSC · 7074-7144 · 71 bp

Back to Pleroma

Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TATCCAATGATCCGGGACGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.3	58.0	185 bp	1.0	78.1
psbI-trnS-GCU_p2	TATCCAATGATCCGGGACGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.3	58.0	186 bp	1.0	78.1
psbI-trnS-GCU_p3	TATCCAATGATCCGGGACGT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.3	58.1	204 bp	1.0	77.2
psbI-trnS-GCU_p4	TATCCAATGATCCGGGACGT	TTTTTCGTACCGAGGGTTCG	50.0	50.0	58.3	58.2	148 bp	1.0	76.5
psbI-trnS-GCU_p5	TATCCAATGATCCGGGACGT	GACTAAAGCGTCGGATTGCT	50.0	50.0	58.3	58.4	183 bp	1.0	75.3