Hotspot

psaC-ndhE

SSC · 120494-121228 · 735 bp

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Pi 0.0194

MarkerSeek score 65.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 735
variable_sites: 62
pi: 0.0194
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	80-1220 bp	1.0	87.2
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	AAGCCGAAGCATGGTTAGAG	50.0	50.0	58.0	58.0	80-1224 bp	1.0	87.1
psaC-ndhE_p3	TTCTCGAGTACGGGCTTTTG	CGAAGCATGGTTAGAGCACT	50.0	50.0	57.9	58.0	80-1357 bp	1.0	86.9
psaC-ndhE_p4	TTCTCGAGTACGGGCTTTTG	AAGCCGAAGCATGGTTAGAG	50.0	50.0	57.9	58.0	80-1361 bp	1.0	86.8
psaC-ndhE_p5	GGACAAGCGGATTCACATCT	CTACGGACTGATCACAAGCC	50.0	55.0	58.0	58.1	80-1239 bp	1.0	86.6