Hotspot

ndhF-rpl32

SSC · 115320-116315 · 996 bp

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Pi 0.0141

MarkerSeek score 71.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 996
variable_sites: 58
pi: 0.0141
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAGCGGAAAAAGGGGTATT	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.9	57.3	80-1142 bp	1.0	57.7
ndhF-rpl32_p2	ACATAGTAACTGGGAGCGGA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	80-1154 bp	1.0	57.6
ndhF-rpl32_p3	TAACTGGGAGCGGAAAAAGG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	80-1148 bp	1.0	57.3
ndhF-rpl32_p4	GGAGCGGAAAAAGGGGTATT	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	57.9	56.2	80-1149 bp	1.0	57.1
ndhF-rpl32_p5	ACATAGTAACTGGGAGCGGA	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	57.8	56.2	80-1161 bp	1.0	56.9