Hotspot

petA-psbJ

LSC · 64028-65062 · 1035 bp

Back to Pleione

Pi 0.0124

MarkerSeek score 64.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1035
variable_sites: 46
pi: 0.0124
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	80-1195 bp	1.0	81.6
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	80-1196 bp	1.0	81.5
petA-psbJ_p3	GGCATCTGTGATTTTGGCAC	ATTCCGGGTTGGGTTCATCT	50.0	50.0	58.0	59.0	80-1194 bp	1.0	79.4
petA-psbJ_p4	GGCATCTGTGATTTTGGCAC	CTCCTATTCCGGGTTGGGTT	50.0	55.0	58.0	59.1	80-1199 bp	1.0	78.7
petA-psbJ_p5	GGCATCTGTGATTTTGGCAC	TCCTATTCCGGGTTGGGTTC	50.0	55.0	58.0	59.1	80-1198 bp	1.0	78.7