Pi
0.0127
MarkerSeek score
65.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGAAAAAGAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 80-666 bp | 1.0 | 84.4 |
| trnT-UGU-trnL-UAA_p2 | AGAAAAAGAGCCGGCTATCG | ACAATCAAGTCCGTAGCGTC | 50.0 | 50.0 | 58.1 | 58.0 | 80-687 bp | 1.0 | 84.3 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 80-615 bp | 1.0 | 84.3 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | ACAATCAAGTCCGTAGCGTC | 55.0 | 50.0 | 57.9 | 58.0 | 80-636 bp | 1.0 | 84.1 |
| trnT-UGU-trnL-UAA_p5 | AGAAAAAGAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 80-674 bp | 1.0 | 83.9 |