Hotspot

trnE-UUC-trnT-GGU

LSC · 32292-33588 · 1297 bp

Back to Pleione

Pi 0.0152

MarkerSeek score 71.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1297
variable_sites: 84
pi: 0.0152
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	80-1534 bp	1.0	87.5
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	80-1513 bp	1.0	87.1
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	80-1510 bp	1.0	86.9
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	80-1512 bp	1.0	86.7
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	80-1531 bp	1.0	86.3