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trnC-GCA-petN

LSC · 28902-29727 · 826 bp

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Pi 0.0115

MarkerSeek score 66.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 41
pi: 0.0115
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCAGTTCGAATCTGGGTGTC	GCTCTAAAGCCCACTCCTTC	55.0	55.0	58.3	58.0	80-970 bp	1.0	85.2
trnC-GCA-petN_p2	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.3	58.2	80-904 bp	1.0	84.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.5	58.0	80-993 bp	1.0	83.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	80-927 bp	1.0	82.3
trnC-GCA-petN_p5	CCAGTTCGAATCTGGGTGTC	CCTTCCCCATACTACAAGCG	55.0	55.0	58.3	57.8	80-954 bp	0.95	81.5