Hotspot

psbK-psbI

LSC · 7779-8256 · 478 bp

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Pi 0.0103

MarkerSeek score 59.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 22
pi: 0.0103
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	80-607 bp	1.0	86.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	80-608 bp	1.0	86.3
psbK-psbI_p3	AATCGTAGACGTTATGCCGG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	80-659 bp	1.0	85.5
psbK-psbI_p4	AATCGTAGACGTTATGCCGG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	80-660 bp	1.0	85.5
psbK-psbI_p5	CGTTATGCCGGTTATCCCTT	ACGCCCCGGATCATTAGATA	50.0	50.0	57.8	58.1	80-650 bp	1.0	84.5