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psbA-trnK-UUU

LSC · 1246-1496 · 251 bp

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Pi 0.0103

MarkerSeek score 61.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 251
variable_sites: 12
pi: 0.0103
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	TCGTGTGCAATGAAAACTGC	50.0	45.0	58.0	57.9	405-424 bp	1.0	82.9
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	AATGAAAACTGCAAGCACGG	50.0	45.0	58.0	57.9	397-416 bp	1.0	82.8
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	CGTGTGCAATGAAAACTGCA	50.0	45.0	58.0	58.2	404-423 bp	1.0	82.5
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	AAAACTGCAAGCACGGTTTG	50.0	45.0	58.0	58.4	393-412 bp	1.0	81.1
psbA-trnK-UUU_p5	GCTTGTACTTTCGCGTCTCT	TCGTGTGCAATGAAAACTGC	50.0	45.0	58.3	57.9	402-421 bp	1.0	80.6