Hotspot

ndhG

SSC · 113384-113914 · 531 bp

Back to Pleioblastus

Pi 0.00105

MarkerSeek score 27.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCATGTTTCGGCTTGTGAT	GCCCGATAGATCTACCCCTT	45.0	55.0	58.1	58.1	1373-1392 bp	1.0	80.6
ndhG_p2	ACCATGTTTCGGCTTGTGAT	ATGCCCGATAGATCTACCCC	45.0	55.0	58.1	58.1	1375-1394 bp	1.0	80.0
ndhG_p3	ACCATGTTTCGGCTTGTGAT	TGTGAAGTATGTGTTCGCGT	45.0	45.0	58.1	57.9	1395-1414 bp	1.0	79.9
ndhG_p4	ACCATGTTTCGGCTTGTGAT	ATTGTGTTGAGTACTGCCCG	45.0	50.0	58.1	58.2	1268-1287 bp	1.0	79.7
ndhG_p5	GCTCTAACCATGTTTCGGCT	GCCCGATAGATCTACCCCTT	50.0	55.0	58.3	58.1	1379-1398 bp	1.0	79.2