Hotspot

ndhA

SSC · 114797-116911 · 2115 bp

Back to Pleioblastus

Pi 0.00281

MarkerSeek score 47.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2115
variable_sites: 23
pi: 0.00281
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2252-2253 bp	1.0	71.2
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2250-2251 bp	1.0	70.7
ndhA_p3	TGAACCCAGTTACCATAGGGA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	57.8	57.9	2255-2256 bp	1.0	70.1
ndhA_p4	TGAACCCAGTTACCATAGGGA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	57.8	57.8	2253-2254 bp	1.0	69.6
ndhA_p5	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2251-2252 bp	1.0	65.5