Hotspot

rbcL-psaI

LSC · 58438-59729 · 1292 bp

Back to Pleioblastus

Pi 0.00473

MarkerSeek score 32.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 11
pi: 0.00473
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CCGGTAGATAAACTAGATAAGT	CCATTGCAATTGCCGGAAAT	36.4	45.0	52.0	58.0	1386-1387 bp	1.0	40.6
rbcL-psaI_p2	CCGGTAGATAAACTAGATAAGT	AAGAAGCCATTGCAATTGCC	36.4	45.0	52.0	57.9	1392-1393 bp	1.0	40.6
rbcL-psaI_p3	CCGGTAGATAAACTAGATAAGTAG	CCATTGCAATTGCCGGAAAT	37.5	45.0	53.4	58.0	1386-1387 bp	1.0	40.6
rbcL-psaI_p4	CCGGTAGATAAACTAGATAAGTAG	AAGAAGCCATTGCAATTGCC	37.5	45.0	53.4	57.9	1392-1393 bp	1.0	40.6
rbcL-psaI_p5	CCGGTAGATAAACTAGATAAGTA	CCATTGCAATTGCCGGAAAT	34.8	45.0	52.1	58.0	1386-1387 bp	1.0	40.6