Hotspot

atpB-rbcL

LSC · 56223-57003 · 781 bp

Back to Pleioblastus

Pi 0.000996

MarkerSeek score 43.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 2
pi: 0.000996
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGATCGATACGCCCTGTG	CCTTGGTTTCATACTCCGGG	50.0	55.0	58.1	58.0	952-954 bp	1.0	79.5
atpB-rbcL_p2	TTTGATCGATACGCCCTGTG	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.1	58.0	951-953 bp	1.0	79.5
atpB-rbcL_p3	TATCCAGCACGGGTCCAATA	CCTTGGTTTCATACTCCGGG	50.0	55.0	58.2	58.0	973-975 bp	1.0	78.4
atpB-rbcL_p4	TATCCAGCACGGGTCCAATA	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.2	58.0	972-974 bp	1.0	78.4
atpB-rbcL_p5	CCAGCACGGGTCCAATAATT	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.2	58.0	969-971 bp	1.0	78.1