Hotspot

ndhC-trnV-UAC

LSC · 52517-53260 · 744 bp

Back to Pleioblastus

Pi 0.00179
MarkerSeek score 43.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
744
variable_sites
3
pi
0.00179
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 877 bp 1.0 75.9
ndhC-trnV-UAC_p2 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.4 57.9 881 bp 1.0 74.8
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.5 58.3 878 bp 1.0 74.1
ndhC-trnV-UAC_p4 TGGTCCTTCACTAATCGGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.8 57.9 880 bp 1.0 73.3
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG CGGTTCGAATCCGTATAGCC 55.0 55.0 57.4 58.3 882 bp 1.0 73.0