Hotspot

rpoC2-rps2

LSC · 31751-32072 · 322 bp

Back to Pleioblastus

Pi 0.00435

MarkerSeek score 46.00

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 322
variable_sites: 4
pi: 0.00435
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ATGAGACATCAGAACCCCCA	ACCAAAATGAACTCCCGCTT	50.0	45.0	58.0	58.0	408-429 bp	1.0	84.5
rpoC2-rps2_p2	TGAGACATCAGAACCCCCAT	ACCAAAATGAACTCCCGCTT	50.0	45.0	58.0	58.0	407-428 bp	1.0	84.5
rpoC2-rps2_p3	ATGAGACATCAGAACCCCCA	TTTACGCTTTGCCGAGATGT	50.0	45.0	58.0	58.2	465-486 bp	1.0	82.6
rpoC2-rps2_p4	TGAGACATCAGAACCCCCAT	TTTACGCTTTGCCGAGATGT	50.0	45.0	58.0	58.2	464-485 bp	1.0	82.6
rpoC2-rps2_p5	TGAGACATCAGAACCCCCAT	CTTTGCCGAGATGTAAGGGG	50.0	55.0	58.0	58.3	458-479 bp	1.0	81.6