Hotspot

trnG-UCC-trnT-GGU

LSC · 14352-16349 · 1998 bp

Back to Pleioblastus

Pi 0.00328
MarkerSeek score 34.60
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1998
variable_sites
21
pi
0.00328
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.65
markerseek_score
34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1390-2085 bp 1.0 45.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1386-2081 bp 1.0 45.0
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1387-2082 bp 1.0 45.0
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1393-2088 bp 1.0 45.0
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1392-2087 bp 1.0 45.0