Hotspot

rpl16

LSC · 85526-86802 · 1277 bp

Back to Plectranthus

Pi 0.0245

MarkerSeek score 58.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1277
variable_sites: 31
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CATATCGGCGATTGCTAGCT	CGACGGAAAAGAAATTGCCC	50.0	50.0	58.0	58.0	1802-1810 bp	1.0	82.3
rpl16_p2	CATATCGGCGATTGCTAGCT	GACGGAAAAGAAATTGCCCG	50.0	50.0	58.0	58.0	1801-1809 bp	1.0	82.3
rpl16_p3	AGCATATCGGCGATTGCTAG	CGACGGAAAAGAAATTGCCC	50.0	50.0	58.0	58.0	1804 bp	0.5	59.8
rpl16_p4	AGCATATCGGCGATTGCTAG	GACGGAAAAGAAATTGCCCG	50.0	50.0	58.0	58.0	1803 bp	0.5	59.8
rpl16_p5	GAGCATATCGGCGATTGCTA	GACGGAAAAGAAATTGCCCG	50.0	50.0	58.0	58.0	1804 bp	0.5	59.8