Hotspot

ndhG-ndhI

SSC · 122196-122563 · 368 bp

Back to Plectranthus

Pi 0.0501

MarkerSeek score 70.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 368
variable_sites: 18
pi: 0.0501
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCCCAATGAAAAGGCCGA	CATGATTACCCTGTCCCACG	45.0	55.0	57.8	58.0	914-920 bp	1.0	80.5
ndhG-ndhI_p2	ATCCCAATGAAAAGGCCGAA	CATGATTACCCTGTCCCACG	45.0	55.0	57.8	58.0	913-919 bp	1.0	80.5
ndhG-ndhI_p3	TCCCAATGAAAAGGCCGAAT	CATGATTACCCTGTCCCACG	45.0	55.0	57.8	58.0	912-918 bp	1.0	80.5
ndhG-ndhI_p4	ATCCCAATGAAAAGGCCGAA	ACATCAGAACGTTTCCGAGG	45.0	50.0	57.8	57.9	846-852 bp	1.0	80.4
ndhG-ndhI_p5	TCCCAATGAAAAGGCCGAAT	ACATCAGAACGTTTCCGAGG	45.0	50.0	57.8	57.9	845-851 bp	1.0	80.4