Hotspot

ccsA-ndhD

SSC · 118807-119035 · 229 bp

Back to Plectranthus

Pi 0.0814

MarkerSeek score 75.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 229
variable_sites: 18
pi: 0.0814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTAATGAAGCATGGGGGTCC	TCAGTAGCTTTTCGATGGCC	55.0	50.0	58.0	58.0	830-831 bp	1.0	83.5
ccsA-ndhD_p2	GAAGCATGGGGGTCCTATTG	TCAGTAGCTTTTCGATGGCC	55.0	50.0	58.0	58.0	825-826 bp	1.0	83.5
ccsA-ndhD_p3	CTAATGAAGCATGGGGGTCC	CAGTAGCTTTTCGATGGCCT	55.0	50.0	58.0	58.0	829-830 bp	1.0	83.4
ccsA-ndhD_p4	GAAGCATGGGGGTCCTATTG	CAGTAGCTTTTCGATGGCCT	55.0	50.0	58.0	58.0	824-825 bp	1.0	83.4
ccsA-ndhD_p5	GAAGCATGGGGGTCCTATTG	GGTTGATAATAGTGGGCGCA	55.0	50.0	58.0	58.0	1122-1123 bp	1.0	82.9