Pi
0.0445
MarkerSeek score
76.10
Primer available
yes
Label
rpl32-trnL-UAG
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl32-trnL-UAG_p1 | TCTCTTTCTACCGGGATTTCA | TCCTAAGAGCAGCGTGTCTA | 42.9 | 50.0 | 56.1 | 57.9 | 855-858 bp | 1.0 | 60.5 |
| rpl32-trnL-UAG_p2 | TCTCTTTCTACCGGGATTTCA | AGCAGCGTGTCTACCAATTT | 42.9 | 45.0 | 56.1 | 57.8 | 848-851 bp | 1.0 | 60.0 |
| rpl32-trnL-UAG_p3 | TCTCTTTCTACCGGGATTTCA | CCTAAGAGCAGCGTGTCTAC | 42.9 | 55.0 | 56.1 | 57.8 | 854-857 bp | 1.0 | 59.9 |
| rpl32-trnL-UAG_p4 | TCTCTTTCTACCGGGATTTCA | CTAAGAGCAGCGTGTCTACC | 42.9 | 55.0 | 56.1 | 57.8 | 853-856 bp | 1.0 | 59.9 |
| rpl32-trnL-UAG_p5 | TCTCTTTCTACCGGGATTTCA | GAGCAGCGTGTCTACCAATT | 42.9 | 50.0 | 56.1 | 58.3 | 849-852 bp | 1.0 | 59.3 |