Hotspot

rpl36-infA

LSC · 84007-84101 · 95 bp

Back to Plectranthus

Pi 0.0526

MarkerSeek score 72.40

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 95
variable_sites: 5
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	AATTACTCTAATGCGCCCCC	TCGACTCCGAAACAAGGATT	50.0	45.0	57.7	56.9	204 bp	1.0	70.8
rpl36-infA_p2	AATTACTCTAATGCGCCCCC	TCGACTCCGAAACAAGGATTT	50.0	42.9	57.7	57.6	204 bp	1.0	68.6
rpl36-infA_p3	AATTACTCTAATGCGCCCCC	CGACTCCGAAACAAGGATTTG	50.0	47.6	57.7	57.5	203 bp	1.0	68.1
rpl36-infA_p4	AATTACTCTAATGCGCCCCC	ATGATTCAACCAGAGGACGT	50.0	45.0	57.7	56.5	232 bp	1.0	67.9
rpl36-infA_p5	AATTACTCTAATGCGCCCCC	CGACTCCGAAACAAGGATTT	50.0	45.0	57.7	56.1	203 bp	1.0	64.4