Hotspot

psbZ-trnG-GCC

LSC · 35811-40756 · 4946 bp

Back to Plectranthus

Pi 0.0943

MarkerSeek score 58.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 4946
variable_sites: 23
pi: 0.0943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGTGATACCAAGGAGAACTGT	ATGCTAGTTCTTGCTCCAGC	42.9	50.0	56.3	58.0	988 bp	0.5	38.0
psbZ-trnG-GCC_p2	TGTGATACCAAGGAGAACTGT	TAGTACGTCGTCCTTCCCTG	42.9	55.0	56.3	58.3	1021 bp	0.5	36.3
psbZ-trnG-GCC_p3	TGTGATACCAAGGAGAACTGT	GTCGTCCTTCCCTGAAATGG	42.9	55.0	56.3	58.3	1015 bp	0.5	36.2
psbZ-trnG-GCC_p4	ACTGTAGAAAGTAAAGGGTGCT	ATGCTAGTTCTTGCTCCAGC	40.9	50.0	57.0	58.0	972 bp	0.5	35.9
psbZ-trnG-GCC_p5	TGTGATACCAAGGAGAACTGT	TCGTCCTTCCCTGAAATGGA	42.9	50.0	56.3	58.3	1014 bp	0.5	35.5