Hotspot

rpl32-trnL-UAG

SSC · 115258-116265 · 1008 bp

Back to Platymiscium

Pi 0.0913

MarkerSeek score 75.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1008
variable_sites: 70
pi: 0.0913
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGACAGCATTGAAAGCGTA	TGGTAAATGGCCCGACAAAA	45.0	45.0	57.8	58.0	1242-1453 bp	1.0	87.0
rpl32-trnL-UAG_p2	TGGACAGCATTGAAAGCGTA	CCGACAAAATCCAGCGAGTA	45.0	50.0	57.8	58.0	1231-1442 bp	1.0	87.0
rpl32-trnL-UAG_p3	AGAGGGGATATTGGACAGCA	TGGTAAATGGCCCGACAAAA	50.0	45.0	57.8	58.0	1253-1464 bp	1.0	86.9
rpl32-trnL-UAG_p4	AGAGGGGATATTGGACAGCA	CCGACAAAATCCAGCGAGTA	50.0	50.0	57.8	58.0	1242-1453 bp	1.0	86.9
rpl32-trnL-UAG_p5	TGGCAGTTCCAAAGAAACGT	TGGTAAATGGCCCGACAAAA	45.0	45.0	58.2	58.0	1527 bp	0.5	58.0