Hotspot

atpA-trnR-UCU

LSC · 52840-53103 · 264 bp

Back to Platymiscium

Pi 0.113

MarkerSeek score 80.70

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 264
variable_sites: 29
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	AAGGAGGCTATTCAGGAGCA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.1	57.3	349-365 bp	1.0	74.9
atpA-trnR-UCU_p2	GGCTATTCAGGAGCAGATGG	GGTTCAAATCCTATTGGACGC	55.0	47.6	57.8	57.3	344-360 bp	1.0	74.5
atpA-trnR-UCU_p3	TCAGGAGCAGATGGAACTCT	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	338-354 bp	1.0	74.2
atpA-trnR-UCU_p4	AAGGAGGCTATTCAGGAGCA	TGGATAGGACAGAGGTCTTCT	50.0	47.6	58.1	56.9	384-400 bp	1.0	71.2
atpA-trnR-UCU_p5	GGCTATTCAGGAGCAGATGG	TGGATAGGACAGAGGTCTTCT	55.0	47.6	57.8	56.9	379-395 bp	1.0	70.8