Hotspot

atpI-atpH

LSC · 48234-49184 · 951 bp

Back to Platymiscium

Pi 0.0396

MarkerSeek score 79.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 33
pi: 0.0396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TACAAGCGGTATTCAAGCCC	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.0	58.0	1011-1062 bp	1.0	86.0
atpI-atpH_p2	TACAAGCGGTATTCAAGCCC	CGCAATCCCTTCTACTGCTT	50.0	50.0	58.0	58.0	1099-1150 bp	1.0	85.8
atpI-atpH_p3	TACAAGCGGTATTCAAGCCC	AAGCAAGTCCTACAGCCAAC	50.0	50.0	58.0	58.1	1040-1091 bp	1.0	85.4
atpI-atpH_p4	TACAAGCGGTATTCAAGCCC	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.0	58.2	1012-1063 bp	1.0	84.8
atpI-atpH_p5	TACAAGCGGTATTCAAGCCC	GAAGCAAGTCCTACAGCCAA	50.0	50.0	58.0	57.8	1041-1092 bp	1.0	84.7