Hotspot

trnF-GAA-trnL-UAA

LSC · 13268-13704 · 437 bp

Back to Platymiscium

Pi 0.0844

MarkerSeek score 82.30

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 33
pi: 0.0844
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TCTACCAGCTGAGCTATCCC	AATCGTGAGGGTTCAAGTCC	55.0	50.0	58.0	57.5	467-491 bp	1.0	85.7
trnF-GAA-trnL-UAA_p2	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	503-527 bp	1.0	84.9
trnF-GAA-trnL-UAA_p3	GTGCCAGGAACCAGATTTGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	518-542 bp	1.0	84.5
trnF-GAA-trnL-UAA_p4	TCAGTCCTCTGCTCTACCAG	AATCGTGAGGGTTCAAGTCC	55.0	50.0	57.9	57.5	479-503 bp	1.0	84.5
trnF-GAA-trnL-UAA_p5	CATGTGCCAGGAACCAGATT	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.2	57.5	521-545 bp	1.0	83.9