Hotspot

ndhJ-trnF-GAA

LSC · 12541-13194 · 654 bp

Back to Platymiscium

Pi 0.0472

MarkerSeek score 77.60

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 29
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	CCCCAATTTTTATGAAATACAAGATGC	CCTCGTGTCACCAGTTCAAA	33.3	50.0	58.2	58.1	690-722 bp	1.0	47.3
ndhJ-trnF-GAA_p2	CCCCAATTTTTATGAAATACAAGATGC	CTGGTAGAGCAGAGGACTGA	33.3	55.0	58.2	57.9	714-746 bp	1.0	47.2
ndhJ-trnF-GAA_p3	CCCCAATTTTTATGAAATACAAGATGC	GGGATAGCTCAGCTGGTAGA	33.3	55.0	58.2	58.0	726-758 bp	1.0	47.1
ndhJ-trnF-GAA_p4	CCCCAATTTTTATGAAATACAAGATGC	GCGTTAAGAATGGTCGGGAT	33.3	50.0	58.2	58.1	741-773 bp	1.0	47.1
ndhJ-trnF-GAA_p5	CCCCAATTTTTATGAAATACAAGATGC	AGAATGGTCGGGATAGCTCA	33.3	50.0	58.2	57.9	735-767 bp	1.0	47.1