Hotspot

trnK-UUU-rbcL

LSC · 4556-5128 · 573 bp

Back to Platymiscium

Pi 0.0702

MarkerSeek score 83.40

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 38
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGAGTCGCACTTAAAAGCC	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.5	58.0	711-746 bp	1.0	83.5
trnK-UUU-rbcL_p2	AGTCGCACTTAAAAGCCGAG	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.6	58.0	708-743 bp	1.0	82.8
trnK-UUU-rbcL_p3	GAGTCGCACTTAAAAGCCGA	GAGGCTAGCAAATGGAGTCC	50.0	55.0	58.6	58.0	709-744 bp	1.0	82.8
trnK-UUU-rbcL_p4	TGGAGTCGCACTTAAAAGCC	GAGTCCTGAGTTAGCTGCTG	50.0	55.0	58.5	57.7	697-732 bp	1.0	81.7
trnK-UUU-rbcL_p5	TCGCACTTAAAAGCCGAGTA	GAGGCTAGCAAATGGAGTCC	45.0	55.0	57.3	58.0	706-741 bp	1.0	81.6